Agricultural Phages Biological Characterization

Agricultural phages have great potential for controlling plant bacterial diseases. They are widespread in nature, can self-replicate, target receptors with pathogenic bacteria, are not toxic to eukaryotes, and at the same time, are specific and do not damage beneficial indigenous flora. Given the isolation and technical development of agrophages, Lifeasible can provide biological characterization tests with a full report.

Lifeasible is dedicated to the biological characterization of agrophages based on isolated and purified phage samples. We offer a complete phage testing program that includes optimal infection complex determination, one-step growth curve determination, chloroform sensitivity determination, UV sensitivity determination, UV sensitivity determination, thermal stability determination, pH stability determination, and storage stability determination.

Services content

• Phage potency determination

Phage potency is the phage titer, the number of phages with infectious activity per milliliter of sample. Our method for phage potency determination usually uses the double-layer plate method.

• Optimal multiplicity of infection assay

The optimal infection multiple is the infection multiple of the offspring phage that gives the highest yield at the end. We take the suspension of R. solanacearum at the logarithmic period and measure the concentration of the suspension, then add the phage according to different infection pluralities, and centrifuge and filter the phage solution for titer determination by bilayer plate method.

• One-step growth curve measurement

The titer was calculated using the double-layer plate method, and the one-step growth curve of the phage was plotted using the logarithm of the phage titer as the vertical coordinate and the incubation time as the horizontal coordinate. The latent period, outbreak period, and lysis amount of the phage were determined based on the one-step growth curve.

• Chloroform sensitivity determination

Agrophage stock solution and quantitative chloroform were mixed, incubated, and phage potency was determined.

• UV sensitivity determination

The agrophage stock solution was irradiated vertically under a UV lamp at different times, and the phage potency was measured.

• Heat stability determination

The phage potency was measured by subjecting the agrophage stock solution to different temperatures.

• Determination of pH stability

The phage potency was determined by subjecting the agricultural phage stock solution to different pH values.

• Determination of storage stability

The phage stock solution is stored at room, low, and high temperatures for a period, and the phage potency is measured periodically.

Feedback to the customer

We provide our customers with complete data reports, experimental protocols, experimental procedures, test conditions, data analysis, etc.

Service process

Advantages

• Efficient service - Our research team and staff can provide efficient service to shorten the project cycle and help you complete your phage development and research as soon as possible.
• Technology as the core - We always take technology as the core and service as the guide, equipped with advanced experimental equipment and professional technical research and development team, to provide customers with professional and reliable technical support.
• All-in-one service - We provide end-to-end service, from phage isolation and purification to phage performance testing, we can provide valid data reports.