Brassica Campestris L. ssp. Chinensis var. Utilis Tsenet Lee Transformation

Brassica Campestris L. ssp. Chinensis var. Utilis Tsenet Lee Transformation

Brassica campestris L. ssp. chinensis var. utilis tsenet lee, also known as flowering cabbage, is a one- or two-year-old herb in the Brassica family of the Cruciferae. It is a widely grown vegetable in all parts of the world. Flowering cabbage has high edible and nutritional value, containing proteins, fats, carbohydrates, minerals, and rich vitamins. Good quality and marketable flowering cabbage usually have high levels of soluble protein, soluble sugar, vitamin C, and low levels of crude fiber. Through genetic transformation, transgenic plants can retain a variety of their original good traits while adding another trait controlled by a target gene. This can provide biological traits such as drought, cold, or insect resistance to the transgenic flowering cabbage, thus enhancing the organism's survival.

Brassica Campestris L. ssp. Chinensis var. Utilis Tsenet Lee Transformation

What We Offer

Lifeasible has been committed to providing you with professional flowering cabbage genetic transformation services that can help advance the process of your related breeding research projects. Our flowering cabbage genetic transformation platform has an efficient experimental process, professional technical service support, and mainstream molecular biology experimental equipment. Based on this, we use Agrobacterium-mediated transformation to provide solutions targeting the improvement of rapeseed traits. First, our experts use the cotyledons of flowering cabbage as the transgenic recipient material and infest the recipient material with Agrobacterium carrying the target gene vector. Subsequently, after resistance screening, we can obtain independently resistant healing tissues. Finally, the healing tissues will be differentiated and regenerated into the target transgenic strains. In addition, we offer a variety of vector structures for you to choose from, as shown below.

Service Flow

Schematic diagram of the standardized process of flowering cabbage genetic transformation. - Lifeasible

1. Sample submission requirements

Customers can provide constructed flowering cabbage transformation vectors directly, and Lifeasible also offers vector construction services.

  • Customer-supplied plasmid requirements
Concentration Volume Other requirements
80-100 ng/μL ≥10 μL no degradation & no contamination
  • Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).
Bacterial broth Bacterial plate
Bacterial broth up to 1-year-old, preserved in glycerol Bacterial plate activated within one week

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our flowering cabbage gene transformation service is equipped with experienced experts throughout the process to facilitate advice and consultation with our customers during the experimental process. We not only keep our customers up to date with the key points of the experiments but also report the results to you regularly. So you will not miss any important stage in the progress of your project. Our one-stop service is designed to reduce wasted time and lower your research costs. We will do as much work as possible, and all you need to do is submit samples as required and wait patiently for the results agreed with us.

3. Brassica campestris L. ssp. chinensis var. utilis tsenet lee transformation results delivery

  • First you will receive the T0 positive seedlings that meet your customized needs.
  • Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
  • Information about test primers, vector sequences, variant genes, etc.
  • Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
  • Complete, detailed, and comprehensive experimental data and reports.

We Do Better

  • Our experienced experts can provide you with one-on-one project consultation, program design, and customization services to ensure that your needs for transforming the dominant traits of your flowering cabbage are met as much as possible.
  • Our transformation platform is based on a universal Agrobacterium-mediated transgenic approach well suited for the efficient and consistent acquisition of superior traits.
  • Our multifunctional platform for gene transformation in flowering cabbage is equipped with efficient experimental procedures, professional pre, and post-sales technical support, and complete molecular biology experimental equipment.
  • Our plant transformation experiments do not have any seasonal restrictions and can be carried out at any time of the year.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in brassica campestris L. ssp. chinensis var. utilis tsenet lee transformation. We can provide customized solutions to help you study a wide range of flowering cabbage varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.

Our products/services are For Research Use Only. Not For Clinical Use!
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