Brassica Campestris var. Purpuraria Transformation

Brassica Campestris var. Purpuraria Transformation

Brassica campestris var. purpuraria, also known as purple cabbage moss, is a genus of Brassica in the cruciferous family. Purple cabbage moss is a high-quality vegetable rich in nutrients, including protein, carotene, ascorbic acid, minerals, and many vitamins. Purple cabbage moss also can be used as medicine to treat postpartum abdominal pain, blood in the stool, and constipation. To further improve the quality and applicability of purple cabbage moss, research on transgenic purple cabbage moss has become a hot research area in recent years. Trait improvement and functional gene research of the purple cabbage moss rely on constructing genetic transformation systems to improve disease resistance, insect resistance, herbicide resistance, salt tolerance, cold tolerance, etc.

Brassica Campestris var. Purpuraria Transformation

What We Offer

Lifeasible offers you a genetic transformation system for purple cabbage moss based on the Agrobacterium transformation method, with the advantages of stability, high transformation efficiency, and short cycle time. The whole custom experimental process is most proven by experts for many years, covering the whole process of experimental design, vector construction, plasmid transformation, positive graft screening, and testing. Agrobacterium-mediated transformation is the optimal method for introducing genes into the purple cabbage moss genome because of its high transformation efficiency and low T-DNA copy integration. With rich experience in plant transformation, we have developed several types of Agrobacterium-mediated genetic transformation methods, such as GV3101, AGL-1, EHA101, EHA105, C58C1, etc. For vector customization, we offer various vector structures, as shown below for your choice.

Service Flow

Schematic diagram of the standardized process of purple cabbage moss genetic transformation. - Lifeasible

1. Sample submission requirements

Customers can provide constructed purple cabbage moss transformation vectors directly, and Lifeasible also offers vector construction services.

  • Customer-supplied plasmid requirements
Concentration Volume Other requirements
80-100 ng/μL ≥10 μL no degradation & no contamination
  • Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).
Bacterial broth Bacterial plate
Bacterial broth up to 1-year-old, preserved in glycerol Bacterial plate activated within one week
  • Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our entire experiment process is open and transparent, and you can contact us at any time to keep abreast of the progress of your experiment. Of course, our staff will also keep you informed when your project reaches a critical point in case you miss it. Our one-stop shop is designed to ease your research burden and save you time so that you can relax and focus on other, more important aspects of your research. When it comes to the genetic transformation breeding of purple cabbage moss, you can leave it to us with complete confidence.

3. Brassica campestris var. purpuraria transformation results delivery

  • First, you will receive the T0 positive seedlings that meet your customized needs.
  • Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
  • Information about test primers, vector sequences, variant genes, etc.
  • Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
  • Complete, detailed, and comprehensive experimental data and reports.

We Do Better

  • We provide one-stop personalized services and are committed to minimizing your research projects and saving time and cost.
  • We use the mainstream Agrobacterium transformation method for purple cabbage moss gene transformation, which has the advantages of high efficiency and stability, ensuring professional and reliable results.
  • We have enough sterile seedlings of common purple cabbage moss species in stock year-round to carry out your gene transformation project at any time.
  • Our purple moss gene transformation projects can be carried out anytime, regardless of species and seasonal restrictions.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Brassica campestris var. purpuraria transformation. We can provide customized solutions to help you study a wide range of purple cabbage moss varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.

Our products/services are For Research Use Only. Not For Clinical Use!
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