Camellia Sinensis Transformation

Camellia sinensis, commonly known as the tea tree, is a shrub of the genus Camellia in the family Camelliaceae. The young leaves of the tea tree can be used to make tea, and the seeds can be used to extract oil. Tea tree essential oil is a potent anti-infective, anti-inflammatory, an antiseptic, antiviral, expectorant, anti-mold, and anti-parasitic agent that can relieve bronchial congestion, dandruff, insect bites, and yeast infections. Tea is rich in organic components, including tea polyphenols, caffeine, proteins, carbohydrates, lipid-like substances, amino acids, aromatic substances, vitamins, and organic acids. In addition, the most inorganic components in tea are P and K, followed by Ca, Mg, Fe, Mn, Al, and S. Tea has both pharmacological and nutritional benefits. With the development of molecular biology, genetic engineering has been increasingly applied to agricultural breeding. Genetic transformation of tea trees can improve traits such as insect resistance, herbicide resistance, and drought tolerance in a short cycle with high efficiency and targeting.

What We Offer

Lifeasible has developed a proven genetic transformation system for tea trees that allows us to provide large-scale and standardized transgenic tea tree services. Our versatile genetic transformation platform can help you efficiently obtain transgenic tea trees that meet your requirements in a short cycle time. We use Agrobacterium transformation to perform targeted changes in dominant traits in tea trees. Experts introduce and integrate exogenous target genes into recipient cells by infiltrating tea tree cotyledons with Agrobacterium tumefaciens carrying the target gene vector. Subsequently, the experts then regenerate the transformed tea tree seedlings by plant tissue culture. Among them, the test to identify whether the target gene is successfully introduced is the key to verifying the success of the genetic transformation of tea trees. Here, we use PCR identification technology for the efficient screening of positive plants. In addition, our various tea tree transgenic vector construction services are listed below.

Service Flow

1. Sample submission requirements

Customers can provide constructed tea tree transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

The whole process of the tea tree genetic transformation service is equipped with a professional team of experts to answer your inquiries and receive your feedback at any time. In the pre-experimental stage, the experts are mainly responsible for professional technical consultation and experimental guidance, as well as understanding the customer's needs to confirm the solution. In the middle of the experiment, the experts will provide stage experimental data and results reporting to facilitate your continuous follow-up of the experiment. After the experiment is completed, we will provide you with positive seedlings that meet your needs within the agreed time. For after-sales issues, you can contact us at any time with any questions you may have.

3. Camellia sinensis transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We provide efficient and short-cycle genetic transformation services for tea trees, covering the whole process of project consultation, experimental design, vector construction, plasmid transformation, and positive plant screening so that time and cost are saved.
• Our platform is based on the widely used Agrobacterium-mediated transformation of tea trees, ensuring transformation success and reliability.
• Our experts have comprehensive knowledge and years of experience to address various technical issues and challenges that arise during tea tree transformation.
• We develop customized solutions to help you study the multiple genotypes and dominant traits of tea trees to ensure that your needs are met.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Camellia sinensis transformation. We can provide customized solutions to help you study a wide range of tea tree varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.