Capsicum Annuum Transformation

Capsicum annuum, also known as chili pepper, is a diploid self-pollinating annual herb belonging to the genus Capsicum in the family Solanaceae. Peppers are native to the Americas and one of the world's oldest vegetable crops. The development of gene sequencing technology and the publication of pepper genome sequences have promoted the application of molecular breeding technology in pepper. Using conventional breeding methods to select highly resistant and high-quality varieties is very difficult and time-consuming, with the result that the genetic diversity of the crop is reduced and beneficial genes are lost. The use of genetic engineering techniques is one of the important methods to improve the yield and quality of pepper.

What We Offer

Lifeasible has been committed to providingproviding one-stop service covering all aspects of experimental design, vector construction, plasmid transformation, positive graft screening, and identification of transgenic peppers. In Lifeasible's pepper gene transformation service, experts use pepper cotyledons as transgenic recipient material, which first needs to be infested with Agrobacterium tumefaciens carrying the target gene vector. T-DNA is then inserted into the genome and screened for resistance in line with the client's needs to obtain independent resistant healing tissues, which are further differentiated and regenerated into transgenic strains. Our various pepper transgenic vector construction services are listed below.

Service Flow

1. Sample submission requirements

Customers can provide constructed chili transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

We will keep our clients informed at key points of the genetic modification experiment. You can also contact the staff any time for continuous follow-up of the experimental process. The success of the experiments will be strictly based on the positive identification of the target genes from the plants. All experiments will be performed by our experienced experts under standardized experimental procedures, following strict QC standards.

3. Capsicum annuum transformation results delivery

• Most importantly, we will deliver the positive seedling that meets your customized needs.
• Identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information on assay primers, vector sequences, mutated genes, etc.
• Experimental data and pictures.
• Complete, detailed, and comprehensive experimental reports.

We Do Better

• Our experimental processes are standardized, efficient, and optimized. For common pepper varieties, we have enough seeds ready for all types of genetic transformation projects to shorten the cycle time. For special pepper varieties, our scalable services also ensure that any of your needs are met.
• We use the Agrobacterium transformation method, which is fast, efficient, stable, single-copy insertion-based, and supports screening for eukaryotic resistance such as Chaumycin, G418, Basta, etc.
• We have a professional technical team engaged in plant genetic transformation for many years, which can guarantee a more successful experiment and shorter period.
• Our pepper breeding service is the best choice for you when you have no time to manage the breeding work or when you cannot breed in special seasons.

Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in capsicum annuum transformation. We can provide customized solutions to help you study a wide range of pepper varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.