Double-stranded RNA Synthesis Service

Double-stranded RNA (dsRNA) induces the silencing of specific genes in vivo, resulting in the RNAi effect. Therefore, it has been widely used in gene function research, agricultural pest control, etc. RNAi is a naturally occurring biological mechanism that regulates gene expression by silencing specific genes by introducing complementary dsRNA molecules. When pests ingest dsRNA molecules, they trigger an RNAi response that degrades the target pest's essential messenger RNA (mRNA), suppressing specific important proteins. Double-stranded RNA, as a key molecule in RNAi-mediated pest control, is required for a wide range of RNAi applications, requiring customized dsRNA synthesis.

Lifeasible is dedicated to insect pest control. We aim to provide various environmentally friendly and efficient solutions to address pest control urgent needs in agriculture and forestry. Currently, we provide efficient dsRNA synthesis solutions that have been effectively utilized in the development of novel RNA biopesticides.

What do we offer?

The synthesis of dsRNA is a critical factor in RNA biopesticides' ability to be applied in the field. Currently, we offer two main methods for efficient dsRNA synthesis.

• Bacterial expression. We use genetic engineering techniques to insert the target gene DNA sequence into a bacterial expression vector. This vector contains appropriate promoters and regulatory elements that enable bacteria to produce the mRNA of the target gene during transcription. It also transcribes the mRNA into ssRNA. This ssRNA can then be utilized to form a double-stranded structure in bacteria through several specific mechanisms to generate dsRNA. Using bacterial expression methods can be utilized to generate dsRNA in large quantities.
• In vitro transcription. Double-stranded RNA templates are recognized and bound by an in vitro transcriptase. This in vitro transcriptase can transcribe the dsDNA template into the corresponding ssRNA. It can also mix the two ssRNA strands together to form the dsRNA. Double-stranded RNA synthesis by in vitro transcription can be used for targeted synthesis of sequence-specific dsRNA fragments.

Advantages of dsRNA custom synthesis in biological control

Our synthesis of customized dsRNAs offers several distinct advantages over traditional chemical pesticides and other biological control methods.

• Target specificity. One of the most significant advantages of double-stranded RNA biopesticides is their superior target specificity. They can be designed to silence specific genes in the target pest. This maximizes the effect on the target pest and reduces harm to non-target organisms.
• Ecological safety. Unlike chemical pesticides that cause harm to the environment, dsRNA biopesticides degrade quickly in the environment, significantly reducing environmental pollution and promoting healthy and sustainable development of the agro-ecosystem.
• Pests will not develop resistance. Double-stranded RNA custom synthesis is developed to target the genes of resistance mechanisms of the target pests, thus delaying or reducing resistance in pest populations.

Our service workflow

Advantages of our services

• High-quality. Our synthesized dsRNA is of stable quality and can be stored for a long time.
• Experienced. We have many years of experience in custom synthesizing dsRNA and have been widely used in many universities and research organizations.
• Fast speed. Gram-level dsRNA can be completed in the shortest time.
• High purity. The dsRNA we synthesize has high purity.

Lifeasible is an industry-leading biotechnology company that provides dsRNA synthesis services to customers worldwide. In dsRNA synthesis customization, we have professional knowledge, rich service experience, advanced technology platform, and we can produce multiple lengths of dsRNA to meet customers’ research projects. If you are interested in us, please feel free to contact us.