Musa Spp. Transformation

Musa Spp., also known as banana, is one of the important food crops in the world. For most banana producing areas, banana production has long suffered from serious threats such as banana leaf blight, heart rot, wilt, banana leaf spot, and banana aphid. These pests and diseases affect plant growth and are the biggest challenges in banana production. Most banana cultivars are triploid, making it difficult to genetically improve them using traditional breeding methods. The rational usage of transgenic technology for the genetic improvement of bananas is particularly important. The Agrobacterium plasmid gene transformation system is the most established technological approach for plant gene transformation pathways which can be applied to a wide range of resistance screening.

What We Offer

Lifeasible is committed to providing efficient one-stop banana genetic transformation services and will be your best partner in rapid banana breeding. We have an experienced banana gene transformation technology team to ensure the professionalism and accuracy of the whole service process. Our versatile banana gene transformation technology platform is mainly based on the Agrobacterium-mediated method with banana leaves as the starting material. After infesting banana leaves with Agrobacterium, the corresponding antibiotic screening is performed. Subsequently, independent resistant healing tissues are obtained and eventually further differentiated and regenerated into transgenic strains. Our various banana transgenic services are listed below for you to choose from.

Service Flow

1. Sample submission requirements

Customers can provide constructed banana transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

The entire banana genetic transformation service is equipped with dedicated experts to provide you with experimental design, experiment customization, experiment operation, project consultation, and progress tracking. Therefore, you can not only contact us at any time to follow up on the progress of your experiments, but we will also inform our clients when the project reaches a critical point. The entire experimental process is standardized and scalable, with the ability to customize individual solutions to meet your needs. All you need to do is to confirm the final experimental protocol in an in-depth discussion with our experts and submit banana samples as required.

3. Musa Spp. transformation results delivery

• First, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate)
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram)
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• We provide efficient one-stop banana genetic transformation services, covering the whole process of experiment design, vector construction, plasmid transformation, positive graft screening, and testing, helping you save valuable research time.
• We can provide year-round genetic transformation experiments for different banana species without needing specific seasons and times.
• We have a constantly optimized and standardized service platform from our team of experts with several years of experience in plant genetic transformation experiments.
• We use the mainstream Agrobacterium transformation method for banana genetic transformation to ensure the professionalism and reliability of the results.

Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in the musa Spp. transformation. We can provide customized solutions to help you study a wide range of banana varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.