Product Name
GFP | Green fluorescent protein (VENUS)
Immunogen
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Background
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Confirmed reactivity
cell lysate overexoressing Venus protein fusion
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl, of sterile water
Expected/apparent MW
26 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 