Product Name
EGFP | Enhanced Green Fluorescence Protein
Immunogen
Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0
Background
EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Purity
Immunogen affinity purified in PBS pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide.
Confirmed reactivity
GFP, EGFP
Reconstitution
For reconstitution add 100 µl of sterile water, ultrapure water
Expected/apparent MW
27 kDa (for GFP without fusion partner)
Tested applications
Immunocytochemistry (ICC), Western blot (WB)
Recommended dilution
1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)
Storage
Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened
Datasheet 