Product Name
EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants
Immunogen
Recombinant, His-tagged EGFP protein from Aequorea victoria, UniProt: P42212
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Purity
Serum. Contains 0.05 % sodium azide.
Confirmed reactivity
EGFP, S65T-GFP, RS-GFP, YFP
Tested applications
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
Assay dependent (IP), 1 : 4000 (IF), 1 : 2000 (WB)
Storage
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 