Product Name
GFP | Green fluorescent protein (VENUS) - Biotin conjugated 40 µg
Immunogen
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Background
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Purity
Immunogen affinity purified serum in PBS pH 7.4, biotin conjugated.
Reactivity
Venus, green fluorescent protein
Confirmed reactivity
VENUS
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
Depends upon fusion partner
Recommended dilution
1 : 1000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Datasheet 