Name: His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)
SKU: APA-AT-052

Product Name

His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

Cat#

APA-AT-052

Host

Mouse

Immunogen

Polyhistidine (HHHHHH) tagged polypeptide

Background

Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.

Format

Lyophilized

Clonality

Monoclonal

Purity

Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.

Quantity

100 µg

Reactivity

Polyhistidine tagged proteins (N- or C-terminus label)

Confirmed reactivity

6xHis tagged proteins

Reconstitution

For reconstitution add 100 µl of sterile water

Expected/apparent MW

depends upon fusion partner

Tested applications

Western blot (WB)

Recommended dilution

1: 5000 - 10 000 (WB)

Storage

Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.

Datasheet