Product Name
His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)
Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
Background
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Purity
Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Reactivity
Polyhistidine tagged proteins (N- or C-terminus label)
Confirmed reactivity
6xHis tagged proteins
Reconstitution
For reconstitution add 100 µl of sterile water
Expected/apparent MW
depends upon fusion partner
Tested applications
Western blot (WB)
Recommended dilution
1: 5000 - 10 000 (WB)
Storage
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Datasheet 