Product Name
His-tag | 3xHis (polyclonal)
Immunogen
KLH-conjugated synthetic peptide 3xHis
Background
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Purity
Antigen affinity purified serum in PBS pH 7.4.
Confirmed reactivity
3xHis tagged proteins
Reconstitution
For reconstitution add 50 µl, of sterile water
Expected/apparent MW
3xHis tagged proteins
Tested applications
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 μg/mL (IF), 1: 1000 - 1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 