Product Name
His-tag | 6xHis (polyclonal)
Immunogen
KLH-conjugated synthetic peptide 6xHis
Background
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Purity
Immunogen affinity purified in PBS pH 7.2.
Confirmed reactivity
6xHis
Predicted reactivity
6xHis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 8000 (ELISA), 1: 1000 (WB)
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 