Product Name
Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - ALP conjugated 40 µg
Immunogen
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Background
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. This antibody is directly conjugated to ALP.
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Reactivity
Cas9 from Streptococcus thermophilus
Confirmed reactivity
Staphylococcus aureus
Predicted reactivity
Staphylococcus pyogenes UniProt: Q99ZW2
Expected/apparent MW
Depends upon a MW of a protein which is a fusion partner.
Recommended dilution
1 : 100 (IL), 1 : 1000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Datasheet 