Product Name
Cas9 | CRISPR-associated endonuclease 9 (polyclonal)
Immunogen
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Background
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Cas9 from Streptococcus thermophilus
Confirmed reactivity
Staphylococcus aureus
Predicted reactivity
Staphylococcus pyogenes UniProt: Q99ZW2
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
Depends upon a MW of a protein which is a fusion partner.
Tested applications
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 100 (IL), 1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 