Product Name
Cas9 | CRISPR-associated endonuclease 9 (monoclonal)
Immunogen
Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Background
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
Purity
Total IgG. Protein A purified in PBS. Contains 0.02 % sodium azide.
Reactivity
Cas9 from Streptococcus pyogenes
Confirmed reactivity
Cas9 from Streptococcus pyogenes
Expected/apparent MW
Depends upon a MW of a protein which is a fusion partner
Tested applications
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 200 (IF), (IP), 1 : 1000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For long term storage -80°C is recommended. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 