Product Name
Rubisco quantitation kit (Western blot)
Host
Primary antibody: Rabbit
Secondary antibody: Goat
Immunogen
KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Cynara cardunculus var altilis, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonana
Predicted reactivity
Alpha proteobacteria, Algae (brown and red), Beta-proteobacteria, Dicots, Conifers, Cryptomonad, Cyanobacteria, Gamma-proeobacteria, Liverworts, Mosses, Prochlorophytes, Pelwitschia
Expected/apparent MW
Rubisco protein standard: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000-1 : 10000 (WB)
Storage
RbcL antibody and protein standard: Please store at -20°C (6 months) or -80°C for long term storage(years). Please avoid freezing and thawing of reconstituted antibodies, make aliquots instead. PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°C and for long term storage (1 year) at -20°C.
Datasheet 