Donkey anti-Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Product Name
Donkey anti-Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Background
Donkey anti-rabbit IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
Purity
Immunogen affinity purified IgG.
Reactivity
Rabbit IgG (H&L)
Recommended dilution
1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Datasheet 