Product Name
Donkey anti-Rabbit IgG (H&L), ALP conjugated
Immunogen
Purified Rabbit IgG
Background
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Purity
Immunogen affinity purified donkey IgG.
Reactivity
Rabbit IgG (heavy and light chains - H&L)
Confirmed reactivity
Rabbit IgG (H&L)
Predicted reactivity
Rabbit IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Tested applications
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC),, 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Storage
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Datasheet 