Product Name
Chicken anti-Rabbit IgG (H&L), HRP conjugated
Immunogen
Purified Rabbit IgG
Background
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Purity
Immunogen affinity purified chicken IgY.
Reactivity
Rabbit IgG (H&L)
Confirmed reactivity
Rabbit IgG (H&L)
Predicted reactivity
Rabbit IgG (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator
Storage
Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Datasheet 