Goat anti-Human IgM (µ chain), HRP conjugated, min, cross-reactivity to human IgA or IgG

Goat anti-Human IgM (µ chain), HRP conjugated, min, cross-reactivity to human IgA or IgG

APA-GA-064

Goat

Goat anti-human IgM (µ chain) HRP conjugated, is a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgA or IgG and affinity purified using solid phase human IgM.

Lyophilized

Polyclonal

Immunogen affinity purified IgG.

1 mg

Human IgM (µ chain)

For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.