Immunogen
Core fucose residues bound to the N-glycan in alpha 1,3
Background
This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Confirmed reactivity
Higher plants, Pheodactylum tricornutum (diatom)
Predicted reactivity
Higher plants, Phaeodactylum tricornutum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
10 - 100 for various glycoproteins
Tested applications
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Recommended dilution
0.5 µg/ml (ELISA), 1 : 40 (IL), 1 ug/10 ml (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 