Immunogen
Recombinant Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530
Background
Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator
Reactivity
Arabidopsis thaliana, Helianthus annuus
Confirmed reactivity
Arabidopsis thaliana, Helianthus annuus
Predicted reactivity
Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 200 µl of sterile water
Expected/apparent MW
47.7 kDa (Arabidopsis thaliana)
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 