Product Name
ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal)
Immunogen
KLH-conjugated peptide derived from available algal and bacterial ADH sequences including Chlamydomonas reinhardtii
Background
Alcohol dehydrogenase (E.C.:1.1.1.1) is an important enzyme for plants and microbes. In microalgae and bacteria the conversion of Acetyl-CoA to ethanol under conditions of oxygen deprivation is catalyzed by the dual function enzyme alcohol/acetaldehyde dehydrogenase (ADH/ALDH; E.C.:1.1.1.1 /1.2.1.10). This reaction results in NAD+ recycling and allows glycolysis to proceed.
Reactivity
C. reinhardtii, E.coli
Confirmed reactivity
Chlamydomonas reinhardtii, E.coli, Streptococcus pmenumoniae
Predicted reactivity
Algae, Nannochloropsis gaditana, Picochlorum sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 200 µl of sterile water
Expected/apparent MW
100 | 100 kDa (C.reinhardtii), 96 kDa (E.coli)
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 