Sesamum Indicum Transformation

Sesamum Indicum Transformation

Sesamum indicum, also known as sesame, belongs to the family Carotaceae and is widely cultivated in many regions of the world. Sesame is an important oilseed crop with high application value. Its seeds contain up to 55% oil. Sesame seeds are rich in nutrients and contain high levels of glycerides, proteins, sugars, and vitamins. Linoleic acid in sesame seeds has the effect of softening blood vessels and regulating cholesterol. Sesame is a plant that is susceptible to biotic and abiotic adversities that affect the yield potential of the crop and improving the crop through modern plant breeding is one of the most important means of overcoming obstacles to sesame yield enhancement. The genetic transformation of sesame will mainly focus on improving disease resistance, insect resistance, herbicide resistance, salt tolerance, and cold tolerance.

Sesamum Indicum Transformation

What We Offer

Lifeasible has a professional and technical team with many years of experience in plant genetic transformation and is committed to providing the most optimized, stable, and shortest cycle time transgenic sesame services. Our versatile sesame genetic transformation platform is based on the Agrobacterium-mediated method. It offers a wide range of Agrobacterium strains (LBA4404, GV3101, EHA105, AGL1, C58, GV2260) and custom-labeled vectors (kanamycin, thaumatin, glufosinate, G418) for you to choose from. This provides customers with more selectivity and can also fully meet various needs. We mainly take care of the whole experimental process, from vector construction to obtaining transgenic plants. You only need to submit samples as required and then wait patiently for the results. In addition, you can choose the vector construction service you need from the links below.

Service Flow

Schematic diagram of the standardized process of sesame genetic transformation. - Lifeasible

1. Sample submission requirements

Customers can provide constructed sesame transformation vectors directly, Lifeasible also offers vector construction services.

  • Customer-supplied plasmid requirements
Concentration Volume Other requirements
80-100 ng/μL ≥10 μL no degradation & no contamination
  • Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)
Bacterial broth Bacterial plate
Bacterial broth up to 1-year-old, preserved in glycerol Bacterial plate activated within one week
  • Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our sesame gene transformation service is staffed by experienced experts throughout the entire process in order to provide practical experimental advice, project consultation, and progress reports during experiments. We will keep you informed when the experiment reaches a critical point in case you miss it. The goal of our one-stop service is to ease your research burden and reduce the waste of valuable research time. We do as much as possible; you only need to submit samples as requested and wait patiently for the results.

3. Sesamum indicum transformation results in delivery

  • First, you will receive the T0 positive seedlings that meet your customized needs.
  • Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
  • Information about test primers, vector sequences, variant genes, etc.
  • Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
  • Complete, detailed, and comprehensive experimental data and reports.

We Do Better

  • Our sesame genetic transformation platform is based on efficient, stable, and mainstream Agrobacterium infestation methods and supports different types of resistance screening.
  • The entire sesame genetic transformation experimental process is standardized and scalable, supporting customized services to meet various needs.
  • Our team of experts has many years of experience in plant genetic transformation research to ensure efficient and successful experimental processes.
  • Our genetic transformation projects are not limited to seasons and varieties, which can be carried out any time after the project protocol is finalized.

Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in sesamum indicum transformation. We can provide customized solutions to help you study a wide range of sesame varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.

Our products/services are For Research Use Only. Not For Clinical Use!
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