Vitis Vinifera L. Transformation

Vitis vinifera L., also known as grape, is a woody vine in the family of Vitaceae. Grapes are famous worldwide as a fruit that can be eaten raw or made into raisins. Grapes can also be used to make wine. In addition, the roots and vines of grapes have medicinal properties that can be used to stop vomiting and calm the fetus. Grapes contain up to 10-30% sugar, mainly glucose. The various fruit acids in grapes help digestion and can strengthen the spleen and stomach. Moreover, grapes are rich in minerals, vitamin B1, vitamin B2, vitamin B6, vitamin C, and vitamin P, and essential amino acids, which are beneficial for neurasthenia and excessive fatigue. Researchers have come to realize that transgenic breeding can greatly compensate for the disadvantages of traditional breeding, such as long breeding cycles and uncertainty. The introduction of genetic transformation tools into the breeding process of grapes can produce new varieties of grapes with high carbohydrates, high yield, herbicide resistance, insect resistance, and drought tolerance.

What We Offer

Lifeasible has a professional grape genetic transformation platform based on professional technical service support and mainstream molecular biology experimental equipment. Moreover, our experts have had many years of research experience in plant genetic transformation, which can ensure the professionalism, stability, and efficiency of the customized transgenic platform service. We use the mainstream Agrobacterium-mediated transformation method to achieve targeted transformation by introducing target genes into the grape genome through Agrobacterium infestation. The successful transformation of grapevine seedlings needs to be identified by PCR positivity, and customers will only receive positive seedlings that meet the requirements in the end. In addition, for vector customization, we offer a variety of vector construction methods, as shown below for your selection.

Service Flow

1. Sample submission requirements

Customers can provide constructed grape transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our grape transgenesis service is staffed with dedicated experts who will provide practical advice, project design, and professional operation throughout the experiment. Our experts will keep you regularly informed of the progress of your experiment to ensure that you can follow up at any time. The entire experimental process is standardized and scalable, with the ability to customize a personalized solution to meet your grapevine trait-targeted transformation needs. All you need to do is to confirm the final experimental protocol in an in-depth discussion with our experts and submit the grape samples as required.

3. Vitis vinifera L. transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• Our experts have many years of experience in plant genetic transformation and comprehensive knowledge of molecular biology to quickly solve technical problems and challenges in grape transformation.
• Adapting to diverse purposes of all customers, multiple Agrobacterium strains (GV3101, AGL-1, EHA101, EHA105, and C58C1) and commercial and customized binary vectors with variant selectable markers (Kanamycin, Hygromycin, Phosphinothricin, G418, etc.) are readily available for your use.
• Our experts will customize solutions for each customer to help them achieve their goals.
• We can provide genetic transformation experiments for different varieties of grapes, not limited to a specific season. Therefore, we can carry out experiments at any time upon receipt of an order.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Vitis vinifera L. transformation. We can provide customized solutions to help you study a wide range of grape varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.