Expression Vector Building Service

Compared with cloning vector construction, expression vector construction may take more time for researchers. Sometimes, the complete ORF may not be expressed, and no recombinant protein can be obtained for downstream experiments. Based on mature molecular cloning technology, Lifeasible can complete expression vector construction quickly and efficiently. All you need is the full sequence of the target gene, clone it into various expression vectors, and select a recombinant plasmid with high expression efficiency. For example, the target gene is constructed into a prokaryotic expression vector (pET series), the structure of the constructed vector is confirmed, and the plasmid is expressed in high concentration in E. coli.

• Mammalian expression vector building service

In the biomedical field, transfection of plasmid vectors into mammalian cells using conventional transfection is the most widely used method. Our vector systems have been optimized for high copy replication in E. coli and high transfection rates into cells. Cells transfected with this vector can be screened or visually tracked based on a user-selected screening marker gene.

• Bacillus subtilis vector building service

We can perform gene optimization synthesis with plasmid construction and transformation screening.

• Overexpression vector building service

Overexpression of target genes is a commonly used means to study gene function. Overexpression plasmids have been widely used in biological research, including gene regulation, protein engineering, recombinant antibody preparation, drug discovery, etc. We ligated the cDNA sequences of target genes into different plasmid vectors and obtained plasmids overexpressing target genes through plasmid construction. By transfection or viral packaging of the target gene, we can realize stable cell lines for overexpression of the target gene. The overexpression plasmids we provide are suitable for the expression of genes in humans, mice, rats, and other species.

• lncRNA and miRNA expression vector building service

IncRNAs and miRNAs are involved in regulating many biological processes and play important roles in various life activities. We can provide you with specific lncRNA and miRNA overexpression vector building, viral packaging, and screening and characterization of stable transient cell lines.

The basic flow of the service

Service delivery content

Plasmid or bacterial solution, sequencing report, SDS-PAGE picture, experiment report.

Sample requirements

• If there is no template, please provide well-preserved sample material that can extract DNA or RNA.
• If there is a template, please provide no less than 1 μg of PCR product, or ≥ 1 μg of genomic DNA, or ≥ 100 ng of plasmid, or ≥10 μl of cDNA sample.
• Provide the sequence or ID of the gene or fragment to be cloned.

Applications

• For the mass expression of a particular gene to obtain a large amount of the target gene product.
• To study the biological function of the target gene product.

Please feel free to contact us with your needs.