Product Name
Rabbit anti-Mouse IgG (H&L), HRP conjugated
Immunogen
Purified Mouse IgG, whole molecule
Background
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Purity
Immunogen affinity purified rabbit IgG.
Reactivity
Mouse IgG (H&L)
Confirmed reactivity
Mouse IgG heavy and light chains (H&L)
Predicted reactivity
Mouse IgG Heavy and Light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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