Product Name
Goat anti-Dog IgM Fc specific, Unconjugated
Immunogen
Purified normal IgM isolated from pooled dog serum
Background
Goat anti-Dog IgM Fc, Unconjugated is a secondary antibody which binds to dog IgM FC part in immunological assays.
Purity
Protein G purified IgG.
Confirmed reactivity
Dog IgM, Fc part
Not reactive in
The antibody does not cross-react with any other component of the dog immunoglobulin system
Reconstitution
For reconstitution add 1 ml of sterile water
Tested applications
immuniprecipitation (IP)
Recommended dilution
Immunoprecipitation, In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells, Precipitin titre 1:64 when tested against pooled normal dog serum in agar-block immunodiffusion titration
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
Datasheet 