Product Name
Goat anti-Dog IgG (H&L), Unconjugated
Immunogen
purified dog normal IgG isolated from pooled dog serum
Background
Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Purity
Protein G purified goat IgG in PBS pH 7.2.
Confirmed reactivity
Dog IgG (heavy & light chains)
Not reactive in
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Tested applications
Immunoprecipitation (IP)
Recommended dilution
Immunoprecipitation, In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells, Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
Datasheet 