Product Name
Goat anti-Dog IgG Fc specific, Biotin conjugated
Immunogen
Purified dog IgG isolated from dog serum.
Background
Goat anti-Dog IgG Fc, Biotin conjugated is a secondary antibody which binds to dog IgG Fc part, in immunological assays.
Format
Lyophilized from PBS pH 7.2
Purity
Protein G purified goat IgG.
Reactivity
Dog IgG Fc specific
Confirmed reactivity
Dog IgG, Fc part
Not reactive in
It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Tested applications
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution
1 : 100-250 (IHC and ICC parrafin sections), 1 : 500-5000 (ELISA), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stored in 4°C up to 7 days. Working dilutions should be stored at 4°C and not frozen. If any precipitate occurs, it should be removed by centrifugation.
Datasheet 