Product Name
Goat anti-Dog IgG (H&L), Biotin conjugated
Immunogen
Purified normal IgG isolated from pooled dog serum
Background
Goat anti-Dog IgG (H&L), Biotin conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Purity
Protein G purified goat IgG in PBS pH 7.2.
Confirmed reactivity
Dog IgG
Not reactive in
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Tested applications
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 10 000 (ELISA), 1 : 100-1 : 500 (ICC, IHC), The optimal working dilution should be established by titration by the end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
Datasheet 