Product Name
Goat anti-Dog serum proteins
Immunogen
Pooled whole dog serum and partly purified serum fractions
Background
Goat anti-Dog serum proteins is a secondary antibody which binds to dog serum proteins, in immunological assays.
Reactivity
Dog serum proteins
Confirmed reactivity
Dog serum proteins
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Tested applications
Immunoprecipitation (IP)
Recommended dilution
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
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Datasheet 