Product Name
GDC-H | H protein of glycine decarboxylase complex (GDC)
Immunogen
purified GDC-H protein from Spinacia oleracea
Background
The Glycine decarboxylase complex (GDC) is abundant in mitochondria matrix of C3 leaves and functions in photorespiratory carbon recovery. GDC enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Reactivity
A. thaliana,P.hybrida cv. Mitchell, P. grandiflora, S. oleracea, T. aestivum, V. faba
Confirmed reactivity
Arabidopsis thaliana, Cyanthobasis fruticulosa, Petrosimonia nigdeensis, Petunia hybrida cv. Mitchell, Portulaca grandiflora, Salsola grandis, Salsola tragus, Spinacia oleracea, Triticum aestivum, Vicia faba
Predicted reactivity
higher plants
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 200 µl of sterile water
Expected/apparent MW
16 kDa
Tested applications
Tissue printing (TP), Western blot (WB)
Recommended dilution
1 : 5 000 (TP), (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.