Product Name
Glucose Colorimetric/Fluorometric Assay Kit
Product Overview
The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.
Description
Glucose (C₆H₁₂O₆; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
Applications
The kit detects 1-10000 μM glucose samples.
Kit Components
• Glucose Assay Buffer
• Glucose Probe (in DMSO)
• Glucose Enzyme Mix (lyophilized)
• Glucose Standard (100 nmol/ml)
Materials Required but Not Supplied
96-well plate with flat bottom
Multi-well spectrophotometer
Detection method
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Compatible Sample Types
Serum, plasma, urine & other body fluids; Growth media; Food
Datasheet 