As a biomaterial, natural spider silk protein fibers have extremely excellent mechanical properties and good biocompatibility. Spider silk mimicry is carried out by genetic technology, and spider silk mimicry mainly involves the construction and expression of recombinant spider silk proteins in exogenous hosts. Lifeasible can provide customers with recombinant spider silk development services, including expression and purification of spider silk proteins, design and construction, and modification of spider silk proteins.
Spider silk has different functions, but different spider silk protein structures are composed of three parts: a non-repetitive N-terminal structural domain, an intermediate repetitive structural domain, and a non-repetitive C-terminal structural domain. To obtain high-performance artificial spider silk materials, recombinant spider silk proteins can be produced by design. The recombinant spider silk proteins were expressed by expression systems, purified to obtain high-purity proteins, and analyzed by circular dichroism (CD) for the secondary structure composition of protein solutions at different pH values. The recombinant spider silk fibers are then prepared by hand drawing, and the mechanical properties of the silk fibers are tested and characterized.
Lifeasible can provide the following services.
| Items | Specification |
| Expression and Purification of Recombinant Spider Silk Proteins | Perform a single multi-repeat module for a particular type of spider silk protein, with the ends of the repeat module containing N-terminal and C-terminal non-repeat regions or a single C-terminal non-repeat region. The repeat modules are combined and repeated to construct plasmids for pure expression in E. coli. |
| Design and Construction Services for Recombinant Spider Silk Proteins | To obtain high-performance artificial spider silk materials, recombinant spider silk proteins were designed and produced. |
| Modification Services for Recombinant Spider Silk Proteins | Recombinant spider silk proteins have distinct repetitive sequences, and each spider silk protein has a different and unique motif, so the motif combinations of each spider silk protein can be fine-tuned and slightly modified to highlight their superior biological properties. |
Expression of recombinant spider silk ins is a highly customized process, and there is no one-size-fits-all solution. Systematic optimization is often required to develop a workable program to express high-quality spider proteins.
Host cells should be carefully selected based on the desired properties.
Vectors are usually optimized for optimal transfection and expression efficiency. In addition to vectors, other conditions used in host cell cultures, such as time and temperature, should be optimized to capture intact target proteins.
Regions characterized by enhanced hydrophobicity, and highly disordered and repetitive amino acid motifs are responsible for protein instability. As long as they are not directly involved in protein function, removing such regions may help to improve protein expression.
Changes in pH, ionic strength, and oxidation state can affect protein stability. This rule of thumb should be considered when seeking the most appropriate buffer formulation during purification and protein storage.
If you are interested in our services, or if you want a service not listed above, please feel free to contact us, and our staff will customize a professional product solution for you.
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