Setaria italica Transformation

Setaria italica is an annual cash crop with physiological characteristics of drought, barren and salinity tolerance, high adaptability, and stable yield. Moreover, Setaria italica is a diploid self-pollinated crop with a small genome, a short growth cycle (50-80 d), and small plants, which makes it an excellent monocotyledonous model plant. With the development of transgenic technology, improving crop yield and quality through genetic transformation and other techniques has become a widely used approach. However, compared with significant crops such as Oryza sativa and Zea mays, the functional gene research of Setaria italica has lagged, and one of the main reasons for this is the slow development of Setaria italica's genetic transformation system. Therefore, it is essential to establish and further optimize the Setaria italica genetic transformation system.

Lifeasible is committed to plant genetic transformation research; we use Setaria italica stem tip as the receptor material, use an Agrobacterium-mediated method to infest the stem tip, and can effectively obtain positive plants. In the field of plant genetic transformation, we aim to establish a set of highly efficient and stable genetic transformation systems by continuously optimizing the conditions of infestation and detection methods to lay the foundation for accelerating the process of Setaria italica transgenic breeding.

What do we offer?

There are many methods for genetic transformation of Setaria italica, including the gene gun method, Agrobacterium-mediated method, PEG-mediated method, ovary injection method and pollen tube passage method. The Agrobacterium-mediated and gene gun methods are the most used methods in Setaria italica genetic transformation. In Setaria italica genetic transformation, the gene gun method can directly introduce exogenous genes into the recipient to transform various tissues and organs. Still, the disadvantages are that the transformation rate is not high, and it is easy to lead to gene silencing and high cost. The disadvantages of Agrobacterium-mediated transformation are low transformation rate, easy gene silencing, and high cost. Agrobacterium-mediated transformation can express the exogenous genes stably and has simple operation and low cost. Usually, we choose the Agrobacterium transformation method more often, in addition to Agrobacterium-mediated Setaria italica genetic transformation receptor materials, including guaiac tissue, young spike, young embryo and mature embryo, etc. However, these receptor materials need to be cultured, and it takes longer to obtain the receptor and has a strong genotype dependence. In addition, we have also utilized the stem tip growth point as the receptor for genetic transformation, which is not limited by the season and can greatly shorten the breeding time.

Our service content and description

We use the healing tissues induced by mature embryos of Setaria italica as the recipient material, which are infiltrated with Agrobacterium carrying the target gene vectors, inserted T-DNA into the genome, screened for antibiotics corresponding to resistance, and further differentiated and regenerated into transgenic strains.

Why choose us?

• We have a professional technical team engaged in plant genetic transformation for many years, which can guarantee the success rate of the experiment.
• We have a highly efficient experimental process. For commonly used Setaria italica varieties, we have sufficient sterile seedlings ready all year round to carry out genetic transformation projects at any time. We adopt the Agrobacterium transformation method, which is fast, efficient, stable, and single-copy insertion-based. At present, we mainly support the screening of eukaryotic resistance such as thiamphenicol, which is recommended to all researchers to choose.
• We have perfect service support. We provide professional technical advice and guidance in the early stage to optimize the project, communicate with each other at any time during the experiment to keep abreast of the progress, and provide the agreed results according to the contract after the completion of the project.

What do customers need to provide?

Our service workflow

Lifeasible has rich experience in providing services in plant genetic transformation research. Relying on our advanced technology platform and professional research team, we aim to accelerate the process of plant genetic transformation research together with researchers. Our Setaria italica transformation service is of great significance to help researchers understand the growth and development of Setaria italica and improve the quality of crops. If you are interested in us, please feel free to contact us.