Bacterial spores are powerful dormant life forms with great resistance. With increased interest in sporozoites, they are widely used for drug and enzyme delivery and novel self-assembling proteins. As a global company focused on bacterial spores research, Lifeasible provides customized solutions for bacterial spores in bioanalytical and biomedical applications. We will provide efficient and cost-effective services to ensure your 100% satisfaction.
Certain bacteria, such as those from the genera Clostridium and Bacillus, can form spores in nutrient-depleted environments. Bacterial spore formation is a process that protects DNA in a hard, dry coating, and these resting (dormant) cells are considered to be the most stable form of life on Earth. Spores can survive in this dormant state for several years, with reports of spores remaining dormant for more than 25 million years. Although spores from different bacteria have similar structures, they differ in shape and size and are specific to the organism in which they develop. Spores have a wide range of industrial applications due to their ease of genetic manipulation, good culture characteristics suitable for large-scale fermentation, superior protein secretion capacity, and generally recognized as safe (GRAS) status. In addition, as a metabolically dormant form of Bacillus subtilis, its spores are of great interest due to their extreme resistance to many environmental stresses, which makes spores a new platform for various applications.
Fig 1. Structure of spores of Bacillus subtilis. (Zhang X, et al., 2020)
The properties of spores provide a stable vehicle for biosensing, drug delivery, and other state-of-the-art bioanalytical applications. Lifeasible serves as a leading developer of bacterial spore solutions. Our goal is to help researchers around the world conduct scientific research on bacterial spores, with a focus on spores for biosensing systems, bioanalysis, display of enzymes, recognition and reporter proteins on surfaces, and biomedical applications.
Our skilled scientists build stable bacterial spore platforms as biological models for studying complex supramolecular structure formation mechanisms and develop their modern biotechnological applications. Our comprehensive solutions for bacterial spores in bioanalytical and biomedical applications include but are not limited to:
Analysis of Bacterial Spores
Lifeasible offers AFM platforms for microbiological studies of bacterial spores In multiple modes, allowing us to follow the dynamic processes associated with spore formation or germination in nutrient cells. We help you obtain imagesof the surface morphology and multilayer structure of spores and their nanomechanical properties, such as elasticity, viscosity, and adhesion.
Detection of Bacterial Spores
Once spores enter human or animal organisms and canned foods, they can germinate and become a source of serious infectious diseases. Lifeasible offers Raman spectroscopy to quickly, highly sensitively, and reliably detect dangerous bacterial endospores in any environmental object along with other biological objects.
Bacterial Spores Surface Display System
Spore surface display is the ideal effect-enhancing, low-cost, and time-consuming technique with potential for environmental, medical, and industrial development applications. Lifeasible develops a novel surface display system based on bacterial spores to display a wide range of bioactive peptides and proteins. Our spore surface display technology platform has been widely used in industry, vaccine development, environment, and peptide library construction.
Bacterial Spores for Enzyme Expression and Delivery
Many specific enzymes are critical for biocatalysis and biotransformation in many industrial processes, and recycling enzymes can effectively reduce costs. Lifeasible offers immobilization of enzymes in spores to improve enzyme recoverability. Our scientists genetically fuse the gene encoding the target enzyme to the gene's N-terminal or C-terminal coding regio encoding the spore shell protein.
Bacterial Spores for Vaccine Expression and Delivery
Mucosal vaccination by oral or intranasal administration can produce an effective immune response. Lifeasible offers services for developing Bacillus subtilis spores for mucosal vaccines, using the spores as a vector for antigen presentation on the spores, primarily through conventional spore presentation techniques and non-recombinant methods.
Bacterial Spores for Single Domain Antibody Production
Single domain antibodies(sdAbs) are widely used as diagnostic and therapeutic reagents in oncology and infectious diseases. Bacillus subtilis does not contain endotoxin and can secrete sdAbs directly into the culture supernatant, which can simplify the downstream purification process. Lifeasible builds stable and efficient Bacillus subtilis expression platforms to develop sdAbs and enable the production of sdAbs according to your needs.
Bacterial Spores for Cancer Drug Delivery
Spores can germinate in the intestine. In addition, drug-carrying spores can target and kill cancer cells at the same time. Lifeasible provides services for developing cancer-targeting spore carriers, enabling the loading of hydrophobic cancer drugs on the spore surface and the release of drugs after germination in the gastrointestinal tract.
Lifeasible continues to explore the potential of bacterial spores as we continue to delve into research and innovation. We are committed to providing professional, customized solutions for bacterial spores to our customers worldwide. We look forward to working with you to discover the fascination of bacterial spores in cooperation. If you are interested in our solutions, please feel free to contact us for more information.
Reference
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