Genome Editing with Zinc Finger Nucleases in Plants

As an expert in genetically modified plants, Lifeasible provides customers with high-quality zinc finger nucleases (ZFNs) gene editing services. Our experts have extensive experience in ZFN gene editing technology and are able to design personalized project solutions for customers.

What Is ZFN?

Zinc finger nucleases (ZFNs), firstly used as artificial restriction enzymes for genome editing, are a class of powerful tools for the addition, deletion or substitution of genetic sequences with much greater precision than traditional plant breeding techniques, exhibiting great prosperous future for crop quality improvement.

ZNF includes a zinc finger DNA-binding domain and a DNA cleavage domain. The DNA-binding domain is usually composed of 3-4 zinc fingers proteins with conjugated Cys2His2 motifs that each recognizes a specific nucleotide triplet based on the residues in their α helix. The DNA cleavage domain is from the FokI restriction endonuclease which must dimerize to cut DNA in a nonsequence-specific manner, and then induces DSBs (DNA double strand breaks). The DSBs are repaired by two pathways: NHEJ (non-homologous end joining), leading to nucleotide insertions, deletions, or nucleotide substitutions in the broken regions, or HDR (homology directed repair), which uses homologous DNA as a template to restore the DSBs allowing for precise and controllable repairs.

Figure 1. DNA recognition and cleavage by ZFNs. (Miller, et al., 2007)

Our Services

Lifeasible has ultra-modern equipment, first-class experts in gene editing and plant science, and we have established close cooperation with renowned companies to provide high quality and short turnaround services.

We offer a variety of zinc finger protein design technologies, such as oligomerized pool engineering (OPEN) system, phage display technology, bacterial one-hybrid, and modular assembly.

Our experts are able to construct heterodimers of FokI domains to improve the specificity of cleavage.

The ZFN services we provide are suitable for a variety of organisms, including Arabidopsis, rice, wheat, alfalfa, corn, cotton, sorghum, soybean, sugarbeet, cabbage, tobacco, eggplant, cucumber, potato, petunia, oilseed rape, etc.

Lifeasible welcomes customers for hands-on help right from the start of their projects. Our tech representatives are happy to provide a thorough walk-through to acquaint the customer with our featured technologies, service plans, collaboration options and more.

Experimental Process of ZFN

Why Choose Lifeasible?