Raphanus Sativus L. Transformation

Raphanus sativus L., also called cherry radish, is a collective name for a number of radish varieties with small fleshy roots that resemble cherries in appearance. Cherry radish contains a variety of nutrients, including various mineral elements, trace elements, vitamins, amylase, glucose, oxidase adenosine, glycine, choline, mustard oil, and essentialin. Cherry radish has a sweet taste and is suitable for raw consumption, which has the effect of promoting gastrointestinal motility, improving appetite, and aiding digestion. The slow progress and long lead time of traditional breeding have severely limited the production and promotion of superior new varieties of cherry radish. With the development and application of genetic modification technology in modern agriculture, there is an opportunity to breed genetically modified cherry radish products with high yield, herbicide resistance, insect resistance, and drought tolerance.

What We Offer

Lifeasible has now developed a mature and standardized plant genetic transformation process that allows us to provide our customers with large-scale and efficient transgenic cherry radish services in a short cycle time. We mainly use the mainstream Agrobacterium transformation method to introduce target genes into the cherry radish genome. Here， we offer various of Agrobacterium strains (LBA4404, GV3101, EHA105, AGL1, C58, GV2260) and selectable vector markers (Kanamycin, Hygromycin, Phosphinothricin, G418) to suit the requirements of different customers. Our versatile cherry radish genetic transformation platform can fully meet various needs and provides customers with more selectivity. In addition, you can select the type of vector construction service you need from the following links

Service Flow

1. Sample submission requirements

Customers can provide constructed cherry radish transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the whole experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

The whole process is standardized and scalable so that our experts have the flexibility to customize and adapt your cherry radish plants for you. During our one-stop service, all you need to do is to have an in-depth discussion with our experts and confirm the final experimental protocol, as well as submit samples upon request. You can always get in touch with our staff to keep track of the experimental stages and processes. This will largely reduce your research burden and save time for other, more important studies. You will feel comfortable and relaxed when you become our partner.

3. Raphanus sativus L. transformation results delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• Our transformation platform is based on efficient, stable, and mainstream Agrobacterium infestation methods to support various trait modification and improvement needs of our customers.
• Our experimental procedures are standardized, efficient, and flexible, thus allowing for expert one-on-one personalization.
• We have a constantly optimized and updated system for transforming cherry radish seedlings, regardless of variety and season.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Raphanus sativus L. transformation. We can provide customized solutions to help you study a wide range of cherry radish varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.