Product Name
Hemin Colorimetric Assay Kit
Product Overview
Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
Description
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ≈ 650 ng/ml) exerting regulatory functions such as repression of nonspecific δ-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces.
Applications
Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
Kit Components
• Hemin Assay Buffer
• Hemin Probe
• DMSO (anhydrous)
• Enzyme Mix
• Hemin Substrate
• Hemin Standard (1 nmol)
Detection method
Absorbance (570 nm)
Datasheet 