Product Name
Phenylalanine Fluorometric Assay Kit
Product Overview
Phenylalanine Assay Kit provides a quick, simple, accurate method for quantifying PHE in biological samples. In the assay, PHE is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Description
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.
Applications
The assay is linear in the range from 0.1 to 1.0 nmol (2-20 μM) of Phenylalanine.
Kit Components
• Phenylalanine Assay Buffer
• Tyrosinase
• Enzyme Mix
• Developer
• Phenyalanine Standard (10.0 µmol)
Materials Required but Not Supplied
Tyrosinase, Enzyme Mix, Developer: Dissolve with 220 µl Assay Buffer separately. Pipette gently to dissolve. Keep on ice. Store at -20°C. Stable for at least two months
Phenylalanine Standard: Dissolve in100 µl dH2O to generate a 10mM solution. Store at -20 °C.
Detection method
Fluorescence (Ex/Em 535/587 nm)
Datasheet 