LBA4404 Chemically Competent Cell
The genotype of LAB4404 Chemically Competent Cell is Ach5 (rif R) Ti pAL4404 (strep R) Octopine. Background of LAB4404 strain is Ach5 type. It contains rifampicin resistant gene (rif) and the carbenicillin resistance gene (carb) as screening label. LAB4404 carries a octoprine-type Ti plasmid pAL4404 to facilitate transformation. Ti plasmid pAL4404 contains vir gene, which is essential for insertion of T-DNA into plant genome. Ti plasmid pAL4404 is disabled to transfer its own T-DNA but enabled to transfer foreign binary vector T-DNA. pAL4404 Ti plasmid contains streptomycin resistance gene.
LBA4404 Chemically Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Store at - 80 °C for 12 months.
LBA4404: 100 μL/tube * 10 tube/50 tube/100 tube.
Transformation efficiency of LBA4404 Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.