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LBA4404 Competent Cell


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LBA4404 Competent Cell

Cat#
ACC-107
Product Name
LBA4404 Competent Cell
Product Overview
The genotype of LBA4404 Competent Cell is Ach5 (rif r) Ti pAL4404 (strepr) Octopine. The background of the LBA4404 strain is Ach5 type. The nuclear gene of LBA4404 contains screening tags - rifampicin resistance gene (rif). In order to facilitate the transformation, the LBA4404 strain carries a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pAL4404 plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly. Since the pAL4404 plasmid contains the screening tag: strep, the LBA4404 strain has streptomycin resistance.
Applications
LBA4404 Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.
Storage
Store at - 80 centigrade for 12 months.
Kit Components
LBA4404: 100μl/tube * 10 tube/50 tube/100 tube; pCAMBIA2301(control vector,10ng/μl): 10μl.
Sensitivity
The conversion efficiency of pCAMBIA2301 plasmid in LBA4404 Competent Cell is >10^4 cfu/μg DNA.
Sample Type
Tomatoes, tobacco and other plants
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