Phyto Cmm Agar Base

Phyto Cmm Agar Base

Cat#
MSM-004
Product Name
Phyto Cmm Agar Base
Form
It contained 0.50g/L of Potassium dihydrogen phosphate, 2.00g/L of Di-potassium hydrogen phosphate, 0.122g/L of Magnesium sulphate anhydrous , 1.50g/L of Boric acid, 0.10g/L of Yeast extract, 10.0g/L of Sucrose and 18.00g/L of Agar with the Final pH7.3 (at 25°C)
Direction
Suspend 32.2 grams in 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize the medium by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add the rehydrated contents of one vial of CNNP supplement. Mix well and pour into sterile Petri plates.
Principle and Interpretation
Bacterial canker is a serious and destructive disease caused by Clavibacter michiganensis subsp. michiganensis in tomatoes and is of major concern due to the great losses to the tomato growing countries.
(1) Symptomatic plants showed reddish brown cavities in the stem, discoloration, and water soaking of vascular tissue. Diseased tissues were washed with phosphate buffer and placed on semi-selective Clavibacter medium (2), yeast extract serves as a nitrogenous source. Boric acid helps in the selectivity of the medium. Phosphates in the medium help in buffering of the medium. Sucrose serves as a carbohydrate source.
Appearance
Light yellow coloured, homogeneous, free flowing powder
Gelling
Firm, comparable with 1.8% Agar gel
Colour and Clarity of prepared medium
Yellow coloured, opalescent gel forms in Petri plates
Reaction
Reaction of 3.22% w/v aqueous solution is pH 7.3 at 25°C
Cultural Response
Cultural characteristics observed with added CNNP supplement, after an incubation at 25-30°C for 6-7 days.
Applications
Semi selective medium for the detection of Clavibacter michiganensis subsp michiganensis on seeds of tomato.
Storage
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
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