Cladosporium cladosporioides Detection Kits

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Cladosporium cladosporioides Detection Kits

Cladosporium cladosporioides PCR Kit is designed for the detection of Cladosporium cladosporioides specific DNA in a real-time PCR.

Cladosporium cladosporioides PCR Kit is designed for the detection of Cladosporium cladosporioides specific DNA in a real-time PCR based on the use of technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Cladosporium cladosporioides specific DNA is based on PCR providing a simple, reliable and rapid result for the detection of Cladosporium cladosporioides. Cladosporium cladosporioides PCR Kit includes a PCR control to monitor for PCR in hibition, and to validate the quality of the sample and the detection result. The Cladosporium cladosporioides PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.

Fungi

1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.

1. MDx 2X PCR Master Mix 350 µL
2. Cladosporium cladosporioides Primer & Probe Mix 70 µL
3. Cladosporium cladosporioides Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1

1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)

Cladosporium is one of the most widespread molds. It includes about 40 species naturally found in soil, on decaying plant material and as plant pathogens. Cladosporium rot (Cladosporium spp.) of grapevine (Vitis vinifera) is a common disease, particularly in Cabernet Sauvignon and other red wine grape cultivars. It is favored by delayed harvest to obtain the phenolic maturity necessary for high-quality red wine. Symptoms appear on mature grapes and are characterized by berry dehydration, a firm decay affecting a small portion of the berry and a superficial olive-green mold. Rapid and accurate detection of Cladosporium infections is highly important to facilitate the monitoring of Cladosporium in plant samples.

PCR

The specificity of Cladosporium cladosporioides PCR Detection Kit is first and foremost ensured by the selection of the Cladosporium cladosporioides-specific primers, as well as the selection of stringent reaction conditions. The Cladosporium cladosporioides primers were checked for possible homologies to all microorganisms in GenBank published sequences by sequence comparison analysis.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.