Product Name
HLVd/LCV/CanCV RT-PCR Kit
Product Overview
HLVd/LCV/CanCV RT-PCR Kit is designed for the detection of Hop Latent Viroid, Lettuce Chlorotic Virus, and Cannabis Cryptic Virus in a single PCR reaction.
Description
The detection of HLVd, LCV, and CanCV specific RNA is based on one-step RT-PCR providing a simple, reliable and rapid result for the detection of HLVd, LCV, and CanCV infection. HLVd/LCV/CanCV TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. This kit contains Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.
Notes
1. Before use, suitable amounts of all components should be completely thawed at room temperature, mixed by gentle vortexing or by pipetting, and centrifuged briefly.
2. Work quickly on ice.
3. The recommended minimum number of DNA samples tested per PCR run is 6.
Warning
1. Follow universal precautions. All specimens should be considered as potentially infectious and handled accordingly.
2. Ensure that a suitable lab coat, disposable gloves, and protective goggles are worn when handling specimens and kit reagents.
3. Use sterile pipette tips with filters. Use proper pipetting techniques and maintain the same pipetting pattern throughout the procedure to ensure optimal and reproducible values.
4. Ensure that appropriate specimen collection, transport, storage, and processing techniques are followed for optimal performance of this test.
Kit Components
1. MDx 2X RT-PCR Master Mix 350 µL
2. HLVd/LCV/CanCV Primer & Probe Mix 70 µL
3. HLVd/LCV/CanCV Positive Control 50 µL
4. Nuclease-Free Water (Negative control) 1.25 mL
5. Product Insert 1
Materials Required but Not Supplied
1. Appropriate Real-Time PCR Instrument with FAM and HEX filter channel
2. DNA Purification Kit
3. Disposable powder-free gloves
4. Benchtop microcentrifuge
5. Micropipettors
6. Sterile pipette tips with filters
7. PCR tubes
8. Vortex mixer
9. PCR reaction preparation station (Optional)
Scientific Background
Multiplexed PCR-based kit for Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV), and Cannabis Cryptic Virus (CanCV) detection. The turn-key solution comes with Master Mix, Primer & Probe Mix, Positive control, and Nuclease free water. Master Mix includes an internal PCR control to monitor PCR inhibition and validate sample quality. Pair withPlant/Fungi RNA Purification Kit for the highest level of sensitivity in detection.
Features & Benefits
The specificity of HLVd/LCV/CanCV TaqMan RT-PCR Kit is first and foremost ensured by the selection of the target-specific primers and probes, as well as the selection of stringent reaction conditions. The target primers were checked for possible homologies to all plant viroids in GenBank published sequences by sequence comparison analysis.
Storage
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.
Datasheet 