Product Name
7-AAD (7-Aminoactinomycin D)
Product Overview
7-Aminoactinomycin D (7-AAD) is used for fluorescence imaging of cell nuclei.
Description
7-Aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon binding to DNA. The 7-AAD/DNA complex can be excited by a 488-nm laser with an emission maximum of 647 nm, which makes this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry.
Usage
Nuclear fluorescent probe
Stability
Stable under normal conditions.
Storage
-20℃, protect from light.
Introduction
7-Aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. Upon binding to DNA, it can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies.
Handling
Always wear recommended Personal Protective Equipment. No special handing advice is required.
Category
Plant Organelle Fluorescent Probes
Excitation/Emission (nm)
546/647
Molecular Formula
C62H87N13O16
Full Name
7-AAD (7-Aminoactinomycin D)
Potential Health Hazard Effects
May cause eye irritation in susceptible persons.
May cause skin irritation in susceptible persons.
May be harmful by inhalation.
May be harmful if swallowed.
Notice
Use the reagent as soon as possible after unpacking!
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Datasheet 