Adenovirus Vector Building Service

Adenovirus is a linear double-stranded DNA envelope-free virus infectious to both dividing and non-dividing cells. Recombinant adenoviral vectors are instrumental viral vectors developed based on adenoviruses, characterized by a wide host range, high immunogenicity, large gene capacity, no integration with the genome, and transient expression. Lifeasible provides first-generation adenovirus (E1/E3-deleted Ad5), chimeric adenovirus, and Gutless adenovirus packaging services. The adenovirus particles are ultra-gradient centrifuged and filtered and titered by chitin immunoassay, making them ideal for use in various experiments.

• Conventional gene overexpression/interference adenovirus
• miRNA sponge/antago adenovirus
• circRNA/lncRNA overexpression and interference adenovirus
• CRISPR/Cas9 adenovirus customization
• Adenovirus purification service

Service flow

Quality assurance

We used the TCID50 method to detect adenovirus titer. The adenovirus was diluted by gradient and then infected 293A cells with 10 wells per gradient, after which the phenomenon of CPE in each well was observed after 8-10 days. The appearance of CPE wells was regarded as positive and the absence of wells was regarded as negative for statistical purposes, and the results were calculated according to the Reed-Muench two-cell method.

• The transfection efficiency of the same cell type under different transfection conditions with the same viral solution can vary by 2-5 times. The difference between different cell types may be even more significant.
• Need qPCR titer to verify the results need to be specified in advance.
• Sterility testing: virus reservoirs inoculated with culture medium have been tested for bacterial and fungal growth.

Common vector selection

Advantages

• Professional vector construction service. Various vectors to meet different customer needs, relying on our molecular cloning platform and more professional service.
• Standard adenovirus packaging process. High viral titer, short cycle time, and good safety.
• Considerate one-stop service. Our professional team completes everything from vector design, construction and synthesis, sequencing and identification to virus packaging, concentration, titer measurement, etc..

Lifeasible develops a program that meets the customer's requirements based on the customer's genetic information and service needs, building adenoviral vectors and packaging viral particles that can be used in cellular experiments and animal experiments. Please feel free to contact us for information.